BD FACSAria The III flow cytometer has many technical patents, excellent multi-color performance, and its simplicity and ease of use have opened the door to complex cell analysis for more researchers, becoming a legendary production in the history of flow cytometry. Its carefully designed liquid flow and optical path system maximizes signal detection. The patented optogel coupled quartz flow cell and octagonal and triangular optical path collection system have the entire system with ultra-high sensitivity and resolution.

BD FACSAria The flow system of the III cell sorter is driven by pressure. Positive air pressure causes the sample cells to flow through a quartz cup flow cell coupled with photoresist. Under the focusing effect of fluid mechanics, the sample particles form a single liquid flow through the flow cell, where the laser beam is orthogonal to the sample flow.

The core part of BD FACSARia III is the quartz cup flow cell, which, together with the laser and the fluorescence collection optical path coupled with the optical gel, forms a truly stable optical path system. In order to achieve higher sensitivity, BD FACSARia III is equipped with the latest generation quartz cup flow cell. Its patented design helps ensure that the laser is precisely focused on the sample stream, producing the strongest signal and collecting the maximum amount of light signal.

The stable optical path minimizes the startup time and improves the reproducibility of the experiment, allowing for automated daily quality control. More importantly, it also improves the efficiency of optical signal collection, with better signal resolution, particularly suitable for the needs of multi-color applications, and even has high sensitivity and resolution of optical signals during high-speed sorting.

In addition to other functions,The design of the latest generation flow cell in BD FACSARia III further improves the resolution of lateral cell and cell DNA cycle analysis.

FACSARia III has excellent analytical performance, extremely high sensitivity, and is equivalent to the performance of the latest technological analytical instruments. This is because it utilizes BD FACSCAN II and BD LSRFortessa The same optoadhesive coupling pool design and stable optical path structure.

The optical structure of this design canAchieve high numerical aperturelightLearn to collect. Flow pool andThe design of the nozzle can reduce the flow rate of particles in the analysis area to collect the maximum light signal, and then accelerate the particles in the liquid flow after passing through the nozzle, thereby performing high-performance cell sorting.

Due toThe precision design of the optical and liquid flow system of BD FACSARia III, compared with traditional air excitation systems at the same analysis and sorting speed, shows that BD FACSARia III has superior optical detection sensitivity.

Different sizes of nozzles enable users to sort sample particles of different sizes. There are four sizes of nozzles to choose from70 microns, 85 microns, 100 microns, and 130 microns. The improved nozzle design makes replacement more convenient and the installation position precise, without deviation. This means that after each insertion of the nozzle, a reproducible droplet morphology can be obtained without the need to readjust the instrument settings, allowing the instrument to have a reproducible setting and calibration state. The software includes specific sorting settings for different nozzles to match the pressure and sorting settings with the nozzle being used.

Innovations in liquid flow systems, such as easy to plug nozzles, automated sorting settings, and easily replaceable filters, make instrument setup more convenient and efficient. The characteristics of liquid flow design include integrated valve combinations and simple and efficient liquid flow pipelines. The software wizard makes sterile sorting settings easier and more efficient. In addition, after the sample runs, both inside and outside the sample collection tube will be automatically flushed to minimize residue and eliminate cross contamination between samples.

For many users, the performance of a high-end flow cytometer depends on its flexibility, which is mainly reflected in the number of parameters that can be detected simultaneously.BD FACSAria III can support six lasers and four independent light spots. Optional lasers include: 633 nm, 561 nm, 488 nm, 445 nm, 405 nm and 375 nm. And it has the ability to simultaneously detect up to 20 parameters and 18 colors, with strong flexibility.

Users can configure instruments based on current and future needs as well as budget constraints. In the future, we can also increase our services by upgrading themNumber of lasers in BD FACSARia III to enhance its capability. In addition, users of BD FACSARia or BD FACSARia II can also obtain new features of BD FACSARia III through the upgrade service.

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Compared with the method of selecting excitation points in the air,The unique and efficient optical system design of BD FACSARia III reduces the cost of use. BD FACSARia III conducts the laser emitted by the solid-state laser through optical fibers, thereby achieving maximum sensitivity and resolution.